The high number of early exits for the British side can be partly explained by tough draws, with six of them coming against seeded players.
Although Samuel may have lost, he delighted the home team with a thrilling five-set match against 15th-seeded Czech and French Open semi-finalist Jakub Mensik.
The 23-year-old wildcard player, ranked a career-high 123rd in the world after reaching the semi-finals in Eastbourne last week, recovered from two sets down to take Mensik to a 10-point tiebreaker.
Samuel struck first in the deciding set and reignited hopes of defeat by coming back to level at five games apiece, but ultimately lost 5-7 6-3 6-3 3-6 7-6 (10-7).
Fellow debutant Wendelken, ranked 202nd in the world, won the first set against Frenchman world number 75 Valentin Royer 4-6, 6-3, 6-3, 6-3.
Harris also managed to win a set against Russian 19th seed Karen Khachanov, but ultimately lost 6-3, 5-7, 6-3, 6-3.
Pinnington Jones played just five games after returning to close out the match against American 28th seed Brandon Nakashima, losing 6-3 7-6 (7-5) 7-5 after trailing 4-3 in the third set when play was suspended late Monday.
In the women’s draw, Boulter also failed to clear the first hurdle, losing 6-2 6-4 to Italian lass Tyra Catherine Grant.
It was Boulter’s first first-round defeat at Wimbledon since 2017 and her first loss in qualifying at a Grand Slam tournament.
It came just 18 days after she scored the biggest win of her ranking career when she defeated world number two Elena Rybakina in a stunning performance at Queen’s.